2015 Fiscal Year Final Research Report
Identification of the candidate genes for Lymphocystis disease resistance in Japanese flounder.
| Project/Area Number |
25292118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ウイルス / 耐病性 / 遺伝子 / ゲノム / 育種 / ヒラメ / マーカー選抜 / SNP |
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| Outline of Final Research Achievements |
Japanese flounder is an economically important aquaculture speceis in Asian countries such as Japan, Korea and China. Lymphocystis disease (LD) is one of important disease and causes serious damage to Japanese flounder aquaculture industry.
In this study, we investigated the candidate genes for Lymphocystis disease resistance in Japanese flounder using linkage analysis, gene expression analysis and sequence analysis. The genetic locus controlling the resistance to lymphocystis disease was localized on 1.1cM (about 134kb) by linkage analysis. Eleven cDNA of candidate genes from the locus controlling the resistance to lymphocystis disease were isolated and used for gene expression analysis and sequence analysis.
Two candidate genes for lymphocystis disease resistance were identified by gene expression analysis and sequence analysis. The genetic frequency of SNP on the candidate genes was about 2% in wild population.
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| Free Research Field |
水族分子遺伝育種学
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