Developments for new tick prevantion tageting Toll signal pathway

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292173
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: TANAKA TETSUYA 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (00322842)
• Co-Investigator(Kenkyū-buntansha): 松尾 智英 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (50383667) ; 辻 尚利 北里大学, 医学部, 教授 (70355171)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: マダニ / Toll様関連因子(TLR) / TRAF関連因子(TRAFD1) / ディフェンシン

Outline of Final Research Achievements

Toll like receptor (TLR) and tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) are very important to protect againt pathogen infection. Therefore, we alalyzed to idetify and characterize TLR and TRAFD1 from the hard tick Haemaphysalis longicornis. Knockdown of TRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, TRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule. On the other hand,recombinant TLR demonstrated growth inhibition activity against B. gibsoni in vitro without a hemolytic effect at used concentration .These results suggested that TLR and TRAFD1 strongly affected innate immunity of ticks.

Free Research Field

節足動物分子工学