Taget molecule of antimicrobial peptides and disease control

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292178
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Tohoku University
• Principal Investigator: Isogai Emiko 東北大学, (連合)農学研究科(研究院), 教授 (80113570)
• Co-Investigator(Kenkyū-buntansha): 福田 智一 東北大学, (連合)農学研究科(研究院), 准教授 (40321640) ; 奥村 一彦 北海道医療大学, 歯学部, 准教授 (60194510)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 抗菌ペプチド / 薬剤耐性菌 / 腫瘍 / メタボローム解析 / アポトーシス

Outline of Final Research Achievements

Antimicrobial peptides (AMPs) are host defense molecules of the innate immune system of all life forms. Recently, they have been characterized as multifunctional peptides.
In this study, we predicted the structure of persulcatusin (IP), which is an AMP derived from the tick. The structural integrity of IP is maintained by the S-S bond. IP showed strong antimicrobial activity against drug-resistant S. aureus but not toxic against mammalian and human cells such as fibroblasts, colon epithelial cells and erythrocytes. In particular against MRSA and VRSA, we found that IP had similar or stronger antimicrobial activity than VCM
We revealed that human cathelicidin antimicrobial peptide and its analogue, suppress the proliferation of colon cancer cells via apoptotic cell death.Large-scale metabolic profiling demonstrated remarkable differences between colon cancer cell lines treated with functional AMP and controls.

Free Research Field

動物微生物学