2015 Fiscal Year Final Research Report
Attempt of generation of disease-specific iPS cells from dog/cat.
| Project/Area Number |
25292187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UMEZAWA AKIHIRO 国立研究開発法人国立成育医療研究センター, 再生医療センター, 副所長/再生医療センター長 (70213486)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ゲノム / iPS細胞 |
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| Outline of Final Research Achievements |
Animal induced pluripotent stem cells (iPSCs) are required for veterinary regenerative medicine. Transformed cells appeared after introducing reprogramming factors into a dog or cat somatic cells, but they did not maintain pluripotency. Comparative metabolome analysis revealed that dog and cat cells were suffered extreme oxidative stress at the initiation of reprogramming. On the other hand, it is successful to make feeder cells from dog and cat somatic cells that can maintain pluripotency of human iPSCs. In addition, the genetic diagnosis of glutaric aciduria type II in cat was established.
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| Free Research Field |
幹細胞生物学
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