Molecular and genetic approach for the drug or microbial resistance of the diamondback moth

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292203
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Yamamoto Kimiko 国立研究開発法人農業生物資源研究所, 昆虫ゲノム研究ユニット, ユニット長 (40370689)
• Co-Investigator(Kenkyū-buntansha): MIYAMOTO KAZUHISA 農業生物資源研究所, 昆虫微生物機能研究ユニット, 上級研究員 (10370686) ; WADA SANAE 農業生物資源研究所, 昆虫微生物機能研究ユニット, 主任研究員 (50414959) ; JOURAKU AKIYA 農業生物資源研究所, 昆虫ゲノム研究ユニット, 主任研究員 (60542115)
• Co-Investigator(Renkei-kenkyūsha): NODA HIROAKI 農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (40343991) ; SATO RYOICHI 東京農工大学, 農学研究科, 教授 (30235428)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: コナガ / ゲノム解析 / 殺虫剤抵抗性 / 連鎖地図

Outline of Final Research Achievements

In this program, we made genome and expression gene analyses of the diamondback moth (Plutella xylostella). Finally we got a lot of fruitful results as below about drug-resistance genes of the moth.
Drug-resistant strains of the diamondback moth were established.The KONAGAbase, a genomic database of the moth, was improved using the additional genomic data of the moth creating by the next-generation sequencing. The linkage analyses of the drug-resistant strains by RAD-Seq analyses strongly suggested that the ABCC2 gene of the moth had a critical role for the Cry1Ac toxin resistance. A point mutation G4946E in the ryanodine receptor gene was concluded the trigger of the flubendiamide resistance in the moth both from Japan and Thailand.
As a result of the trial experiments to create fungus-resistant strains of the moth, the sensitivity differences for the entomopathogenic fungus B. bassiana were found between several diamondback moth strains.

Free Research Field

分子生物学