Photocontrol of NO and H2S treatment as signaling molecules and biological applications

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Nagoya City University
• Principal Investigator: Nakagawa Hidehiko 名古屋市立大学, 薬学研究科(研究院), 教授 (80281674)
• Co-Investigator(Renkei-kenkyūsha): Ieda Naoya 名古屋市立大学, 大学院薬学研究科, 助教 (00642026) ; Miyata Naoki 名古屋市立大学, 大学院薬学研究科, 特任教授 (50114674)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ケミカルバイオロジー / ケージド化合物 / 薬学 / 有機化学 / 光スイッチ

Outline of Final Research Achievements

A caged NO molecule, Flu-DNB-DB, was developed and applied for cultured cells. When the cells loaded with Flu-DNB-DB was irradiated with near infrared femto-second pulse laser, NO release within the irradiated cells was observed. Another caged NO molecule, Rol-DNB, was developed. Rol-DNB was found to release NO upon photo irradiation at the range of 530-590 nm, yellow-green light, with spatiotemporal resolution. Rol-DNB was also found to be localized to mitochondria within the cells. It was demonstrated that NO release from Rol-DNB upon yellow-green light resulted in the enhancement of mitochondrial fragmentation depending on Drp-1 activation.

Free Research Field

創薬化学、ケミカルバイオロジー