Roles of heparan sulfate modification in biological signaling

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: Masu Masayuki 筑波大学, 医学医療系, 教授 (20243032)
• Co-Investigator(Kenkyū-buntansha): KEINO-MASU Kazuko 筑波大学, 医学医療系, 講師 (50344883)
• Co-Investigator(Renkei-kenkyūsha): NAGATA Michio 筑波大学, 医学医療系, 教授 (10192238)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ヘパラン硫酸 / 糖鎖 / スルファターゼ / ノックアウトマウス / 脳 / 腎臓 / 糖尿病

Outline of Final Research Achievements

We performed analysis of heparan sulfate endosulfatases, Sulf1 and Sulf2, in biological signaling by using knockout mice. The double knockout mice showed corticospinal tract defects, including defects in midline crossing at the pyramidal decussation and abnormal trajectory towards the superior colliculus. The double knockout mice also showed slight abnormalities in kidney glomeruli, especially in the mesangial region. Furthermore they showed severer glomerular abnormalities after inducing diabetes mellitus by streptozotocin compared with the wild-type controls.

Free Research Field

神経科学