Research on host factors which are involved in the progression of HCV-induced hepatic deseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Okayama University
• Principal Investigator: KATO NOBUYUKI 岡山大学, 医歯(薬)学総合研究科, 教授 (40150883)
• Co-Investigator(Kenkyū-buntansha): IKEDA Masanori 鹿児島大学, 大学院医歯(薬)学総合研究科, 教授 (30315767) ; DANSAKO Hiromichi 岡山大学, 医歯(薬)学総合研究科, 助教 (80379841) ; SATOH Shinya 岡山大学, 医歯(薬)学総合研究科, 助教 (80333558)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: C型肝炎ウイルス / 長期継代培養 / 遺伝子発現変動 / miRNA発現変動 / Exosome / ヒト不死化肝細胞 / RNA複製増殖 / マイクロアレイ解析

Outline of Final Research Achievements

We studied to clarify the relation of long-term replication of HCV RNA and hepatocarcinogenesis, and then obtained the following results. (1) Regarding CPB2 and BASP1 genes, whose expression levels were remarkably suppressed by long-term replication of HCV RNA, we analyzed the molecular mechanism of the gene suppression, and partly elucidated it. (2) We found that miR-22 and miR-34a were significantly suppressed by long-term replication of HCV RNA. (3) We developed the reproducible and stable method that we could prepare exosome derived from human hepatocytes, and then we applied this method to the variability analysis of exosome in the long-term culture of HCV RNA-replicating cells. (4) Using immortalized human hepatocytes, we tried the development of a new model of HCV RNA-replicating cells, and then obtained the useful knowledge for future study.

Free Research Field

基礎医学