Altered expression of miRNA and gene expression in lungs of pulmonary arterial hypertension

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: University of Tsukuba
• Principal Investigator: SAKAI SATOSHI 筑波大学, 医学医療系, 講師 (30282362)
• Co-Investigator(Kenkyū-buntansha): BUKAWA Hiroki 筑波大学, 医学医療系, 教授 (80173558) ; SUMIDA Takayuki 筑波大学, 医学医療系, 教授 (00183054) ; MIYAUCHI Takashi 筑波大学, 医学医療系, 教授 (60222329) ; WARABI Eiji 筑波大学, 医学医療系, 講師 (70396612)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マイクロRNA / 肺高血圧症 / マイクロアレイ

Outline of Final Research Achievements

We analyzed the expression of miRNA and mRNA in lungs of patients with pulmonary arterial hypertension (PAH) (n=3) and normal subject (n=3) using the microarray chips for miRNA and mRNA. We obtained actually decreased 81 genes among the target genes predicted from the increased miRNA in PAH lungs. These decreased 81 genes is involved in TGFb signaling pathway (ALK1, BMP2, BMPR2) and cytotoxic responses (FAS, ICAM1). It suggests that the suppression of TGFb signaling and anti-apoptosis contributes to the development of pathogenesis of PAH. Moreover, decrease in ALK1, BMP2, BMPR2 expression and inhibition of apoptosis by decreased FAS expression are partly regulated by miRNA augmentation in lungs of patients with PAH.

Free Research Field

循環器内科学