Research of neutralizing antibody recognizing glycopeptides onto hepatitis C virus and basic studies for new diagnoses

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293134
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Shimizu Hiroki 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30344716)
• Co-Investigator(Kenkyū-buntansha): 奥田 徹哉 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20443179) ; 鈴木 哲朗 浜松医科大学, 医学部, 教授 (00250184) ; 松岡 浩司 埼玉大学, 理工学研究科, 教授 (40272281) ; 松尾 一郎 群馬大学, 大学院理工学府, 教授 (40342852)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: Ｃ型肝炎 / 糖ペプチド / 糖鎖 / 抗体

Outline of Final Research Achievements

The aim of this project was research of neutralizing antibody recognizing glycopeptides onto hepatitis C virus (HCV) and basic studies for new diagnoses.
We tried to produce a neutralizing antibody which recognize glycopeptide in E2 glycoprotein onto HCV. The first stage, to synthesize glycopeptide antigen having developed (by Okuda et al) immunization tag, faced many difficulties, but finally, we achieved to synthesize the designed glycopepitde antigen with aid of new microwave techniques which Shimizu et al developed. Then, immunization was performed and currently produced antibodies are evaluated.

Free Research Field

有機合成化学