Neoatherosclerosisの本態解明と新規治療法の開発

2014 Fiscal Year Annual Research Report

• Project/Area Number: 25293182
• Research Institution: Kyoto University
• Principal Investigator: 木村 剛 京都大学, 医学(系)研究科(研究院), 教授 (80359786)
• Co-Investigator(Kenkyū-buntansha): 尾野 亘 京都大学, 医学(系)研究科(研究院), 准教授 (00359275) ; 今井 逸雄 京都大学, 医学(系)研究科(研究院), 助教 (10625677) ; 齋藤 成達 京都大学, 医学(系)研究科(研究院), 助教 (20467484) ; 西 英一郎 京都大学, 医学(系)研究科(研究院), 准教授 (30362528) ; 牧山 武 京都大学, 医学(系)研究科(研究院), 助教 (30528302) ; 塩井 哲雄 京都大学, 医学(系)研究科(研究院), 講師 (50360095)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: neoatherosclerosis / 血栓 / ステント

Outline of Annual Research Achievements

①BMSおよびDES留置を行った症例でのステント治療部位の解析：ステント留置後の長期フォロー例を中心にデータの蓄積を行い、これらの症例サンプルにおいて詳細な基礎的検討を行っている。
②BMSおよびDES留置後、VLSTを発症した患者の吸引血栓の病理的分子生物学的検討：17例の早期血栓症（early stent thrombosis; EST 30日以内の発症）、7例の遅発性血栓症 (late ST; LST 31-365日での発症)、24例の超遅発性血栓症 (very late ST; VLST 1年以降の発症)での血栓の性状をさらに検討した。血栓の中の好酸球分画はVLSTでは8.2±5.7%、LSTでは5.5±3.8%、EST4.3±3.0%と、有意にVLSTに多かった。また、血管造影上peri-stent contrast staining (PSS)と呼ばれる所見あるいはstentの不完全な留置を得られた例においては、好酸球分画が有意に高かった。さらにVLSTの病理組織内において、13%に動脈硬化巣の組織の一部を認め、neoatherosclerosisがVLSTの原因をなしている可能性が示唆された。
③ステント留置後の剖検症例の集積：症例を増加させている。
④血管内皮を早期に増殖、障害血管の再内皮化をはかるステントの開発：早期の臨床応用を視野に、ウイルスなどのベクターを使用せず、miR-126を局所で過剰発現させるための2本鎖合成RNAを設計した。さらに、この2本鎖合成RNAを乳酸・グリコール酸共重合体（PLGA）ナノ粒子に含有させ、ステントに電気的に均一にコーティングし、局所で徐放化できるステントを開発した。
まずこのステントの効果をウサギの腸骨動脈を用いて検証した。miR-126搭載ステントにおいて、コントロールRNAと比較して、有意に再狭窄予防効果を認めた。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

①BMSおよびDES留置を行った症例でのステント治療部位の解析：VLSTの頻度は0.1%と非常に低いが、着実に症例を集積している。
②BMSおよびDES留置後、VLSTを発症した患者の吸引血栓の病理的分子生物学的検討：17例の早期血栓症（early stent thrombosis; EST 30日以内の発症）、7例の遅発性血栓症 (late ST; LST 31-365日での発症)、24例の超遅発性血栓症 (very late ST; VLST 1年以降の発症)での血栓の性状をさらに検討し、疾患の本態解明に近づいている。
③ステント留置後の剖検症例の集積：冠動脈病理研究レジストリーで集積された病理標本から臨床症例を集積している。
④血管内皮を早期に増殖、障害血管の再内皮化をはかるステントの開発：miR-126の徐放化ステントをウサギモデルに用い、良好な効果を認めた。

Strategy for Future Research Activity

ステントによる冠動脈治療は虚血性心疾患に対する標準的な治療である。薬剤溶出性ステント（Drug eluting stent; DES）の登場により治療後早期の再狭窄率も劇的に改善した。しかしながらDES留置後1年以降に生じる超遅発性ステント血栓症（very late stent thrombosis; VLST）と遠隔期に生じるステント内再狭窄であるlate catch-up現象が重大な問題として残されている。これらの共通の原因としてステント治療後のneoatherosclerosisが注目されているが、この病態解明と治療へ向けて、臨床症例の解析と新規ステントの開発を進めていく。

Research Products

• [Journal Article] MicroRNA-33a/b in lipid metabolism – novel “thrifty” models. (2015)
  • Author(s): Ono K, Horie T, Nishino T, Baba O, Kuwabara Y, Yokode M, Kita T, Kimura T.
  • Journal Title: Circ J. Volume: 79 Pages: 278-84
  • DOI: 10.1253/circj.CJ-14-1252.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In vitro assessment of physiological impact of recipient artery intervention on the contralateral donor artery. (2015)
  • Author(s): Watanabe H, Saito N, Matsuo H, Kawase Y, Watanabe S, Bao B, Yamamoto E, Higami H, Nakatsuma K, Kimura T.
  • Journal Title: Cardiovasc Revasc Med. Volume: 16 Pages: 90-100.
  • DOI: 10.1016/j.carre.2015.01.003.
  • Open Access / Acknowledgement Compliant
• [Journal Article] Relationships between nutritional status and markers of congestion in patients with pulmonary arterial hypertension. (2015)
  • Author(s): Kawamoto A, Kato T, Minamino-Muta E, Okano Y, Shioi T, Kimura T.
  • Journal Title: Int J Cardiol. Volume: 187 Pages: 27-28.
  • DOI: 10.1016/j.ijcard.2015.03.354.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical impact of left ventricular outflow tract obstruction in takotsubo cardiomyopathy. (2015)
  • Author(s): Kawaji T, Shiomi H, Morimoto T, Tazaki J, Imai M, Saito N, Makiyama T, Shizuta S, Ono K, Kimura T.
  • Journal Title: Circ J. Volume: 79 Pages: 839-46.
  • DOI: 10.1253/circj.CJ-14-1148.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Five-Year Outcomes of Percutaneous Versus Surgical Coronary Revascularization in PatientsWith Diabetes Mellitus (from the CREDO-Kyoto PCI/CABG Registry Cohort-2). (2015)
  • Author(s): Marui A, Kimura T, Nishiwaki N, Mitsudo K, Komiya T, Hanyu M, Shiomi H, Tanaka S, Sakata R; CREDO-Kyoto PCI/CABG Registry Cohort-2 Investigators.
  • Journal Title: Am J Cardiol. Volume: 115 Pages: 1063-72.
  • DOI: 10.1016/j.amjcard.2015.01.544.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intravascular ultrasound observation of an obstruction of the left main coronary artery caused by displaced leaflet calcification and hematoma after transcatheter aortic valve implantation. (2015)
  • Author(s): Taniguchi T, Saito N, Minakata K, Imai M, Watanabe H, Toyota T, Watanabe S,Tazaki J, Koizumi S, Hirao S, Yamazaki K, Daijo H, Sakata R, Kimura T.
  • Journal Title: Circulation. Volume: 131 Pages: e345-6
  • DOI: 10.1161/CIRCULATIONAHA.114.014690.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The organ-protective effect of N-type Ca(2+) channelblockade. (2015)
  • Author(s): Kuwahara K, Kimura T.
  • Journal Title: Pharmacol Ther. Volume: 15 Pages: 00045-5
  • DOI: 10.1016/j.pharmthera.2015.02.001.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Measurement of technetium-99m sestamibi signals in rats administered a mitochondrial uncoupler and in a rat model of heart failure. (2015)
  • Author(s): Kawamoto A, Kato T, Shioi T, Okuda J, Kawashima T, Tamaki Y, Niizuma S,Tanada Y, Takemura G, Narazaki M, Matsuda T, Kimura T.
  • Journal Title: PLoS One. Volume: 10 Pages: e0117091
  • DOI: 10.1371/journal.pone.0117091.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of radiofrequency catheter ablation of persistent atrial fibrillation on the left atrial function: assessment by 320-row multislice computed tomography. (2015)
  • Author(s): Hanazawa K, Kaitani K, Hayama Y, Onishi N, Tamaki Y, Miyake M, Kondo H,Tamura T, Izumi C, Shizuta S, Kimura T, Nakagawa Y.
  • Journal Title: Int J Cardiol. Volume: 179 Pages: 449-54
  • DOI: 10.1016/j.ijcard.2014.11.103.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MicroRNA-451 exacerbates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy in mice through suppression of the LKB1/AMPK pathway. (2015)
  • Author(s): Kuwabara Y, Horie T, Baba O, Watanabe S, Nishiga M, Usami S, Izuhara M, Nakao T, Nishino T, Otsu K, Kita T, Kimura T, Ono K.
  • Journal Title: Circ Res. Volume: 116 Pages: 279-88
  • DOI: 10.1161/CIRCRESAHA.116.304707.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Outcomes of everolimus-eluting stent incomplete stent apposition: a serial optical coherence tomography analysis. (2015)
  • Author(s): Shimamura K, Kubo T, Akasaka T, Kozuma K, Kimura K, Kawamura M, Sumiyoshi T, Ino Y, Yoshiyama M, Sonoda S, Igarashi K, Miyazawa A, Uzui H, Sakanoue Y, Shinke T, Morino Y, Tanabe K, Kadota K, Kimura T.
  • Journal Title: Eur Heart J Cardiovasc Imaging. Volume: 16 Pages: 23-8
  • DOI: 10.1093/ehjci/jeu174.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cost analysis of non-invasive fractional flow reserve derived from coronary computed tomographic angiography in Japan. (2015)
  • Author(s): Kimura T, Shiomi H, Kuribayashi S, Isshiki T, Kanazawa S, Ito H, Ikeda S, Forrest B, Zarins CK, Hlatky MA, Norgaard BL.
  • Journal Title: Cardiovasc Interv Ther. Volume: 30 Pages: 38-44
  • DOI: 10.1007/s12928-014-0285-1.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparison of long-term mortality after acute myocardial infarction treated by percutaneous coronary intervention in patients living alone versus not living alone at the time of hospitalization. (2015)
  • Author(s): Nakatsuma K, Shiomi H, Watanabe H, Morimoto T, Taniguchi T, Toyota T, Furukawa Y, Nakagawa Y, Horie M, Kimura T; CREDO-Kyoto AMI Investigators.
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 522-7
  • DOI: 10.1016/j.amjcard.2014.05.029.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CREDO-Kyoto PCI/CABG Registry Cohort-2 Investigators. Effect of statin therapy on cardiovascular outcomes after coronary revascularization in patients ≥ 80 years of age: observations from the CREDO-Kyoto Registry Cohort-2. (2014)
  • Author(s): Natsuaki M, Morimoto T, Furukawa Y, Shiomi H, Ono K, Kimura T
  • Journal Title: Atherosclerosis. Volume: 237 Pages: 821-8
  • DOI: 10.1016/j.atherosclerosis.2014.10.108.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association of localized hypersensitivity and in-stent neoatherosclerosis with the very late drug-eluting stent thrombosis. (2014)
  • Author(s): Yamaji K, Kubo S, Inoue K, Kadota K, Kuramitsu S, Shirai S, Ando K, Nobuyoshi M, Mitsudo K, Kimura T.
  • Journal Title: PLoS One. Volume: 25 Pages: e113870.
  • DOI: 10.1371/journal.pone.0113870.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Serofendic acid protects against myocardial ischemia-reperfusion injury in rats. (2014)
  • Author(s): Ioroi T, Akao M, Iguchi M, Kato M, Kimura T, Izumi Y, Akaike A, Kume T.
  • Journal Title: J Pharmacol Sci. Volume: 126 Pages: 274-80
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cardiac-specific inhibition of kinase activity in calcium/calmodulin-dependent protein kinase kinase-β leads to accelerated left ventricular remodeling and heart failure after transverse aortic constriction in mice. (2014)
  • Author(s): Watanabe S, Horie T, Nagao K, Kuwabara Y, Baba O, Nishi H, Sowa N, Narazaki M, Matsuda T, Takemura G, Wada H, Hasegawa K, Kimura T, Ono K.
  • Journal Title: PLoS One. Volume: 9 Pages: e108201.
  • DOI: 10.1371/journal.pone.0108201.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of preinfarction angina pectoris on long-term survival in patients with ST-segment elevation myocardial infarction who underwent primary percutaneous coronary intervention. (2014)
  • Author(s): Taniguchi T, Shiomi H, Toyota T, Morimoto T, Akao M, Nakatsuma K, Ono K, Makiyama T, Shizuta S, Furukawa Y, Nakagawa Y, Ando K, Kadota K, Horie M, Kimura T.
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 1179-86
  • DOI: 10.1016/j.amjcard.2014.07.038.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparison of five-year outcomes of coronary artery bypass grafting versus percutaneous coronary intervention in patients with left ventricular ejection fractions≤50% versus >50% (from the CREDO-Kyoto PCI/CABG Registry Cohort-2). (2014)
  • Author(s): Marui A, Kimura T, Nishiwaki N, Mitsudo K, Komiya T, Hanyu M, Shiomi H, Tanaka S, Sakata R; CREDO-Kyoto PCI/CABG Registry Cohort-2 Investigators.
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 988-96.
  • DOI: 10.1016/j.amjcard.2014.07.007.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Inhibition of N-type Ca2+ channels ameliorates an imbalance in cardiac autonomic nerve activity and prevents lethal arrhythmias in mice with heart failure. (2014)
  • Author(s): Yamada Y, Kinoshita H, Kuwahara K, Nakagawa Y, Kuwabara Y, Minami T, Yamada C, Shibata J, Nakao K, Cho K, Arai Y, Yasuno S, Nishikimi T, Ueshima K, Kamakura S, Nishida M, Kiyonaka S, Mori Y, Kimura T, Kangawa K, Nakao K.
  • Journal Title: Cardiovasc Res. Volume: 104 Pages: 183-93
  • DOI: 10.1093/cvr/cvu185.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Incidence and outcome of surgical procedures after coronary artery bypass grafting compared with those after percutaneous coronary intervention: a report from the Coronary Revascularization Demonstrating Outcome Study in Kyoto PCI/CABG Registry Cohort-2. (2014)
  • Author(s): Tokushige A, Shiomi H, Morimoto T, Ono K, Furukawa Y, Nakagawa Y, Kadota K, Ando K, Shizuta S, Tada T, Tazaki J, Kato Y, Hayano M, Abe M, Hamasaki S, Ohishi M, Nakashima H, Mitsudo K, Nobuyoshi M, Kita T, Imoto Y, Sakata R, Okabayashi H, Hanyu M, Shimamoto M, Nishiwaki N, Komiya T, Kimura T
  • Journal Title: Circ Cardiovasc Interv. Volume: 7 Pages: 482-91.
  • DOI: 10.1161/CIRCINTERVENTIONS.113.001056.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Predictive Performance of SYNTAX Score II in Patients With Left Main and Multivessel Coronary Artery Disease-analysis of CREDO-Kyoto registry. (2014)
  • Author(s): Campos CM, van Klaveren D, Iqbal J, Onuma Y, Zhang YJ, Garcia-Garcia HM, Morel MA, Farooq V, Shiomi H, Furukawa Y, Nakagawa Y, Kadota K, Lemos PA, Kimura T, Steyerberg EW, Serruys PW.
  • Journal Title: Circ J. Volume: 78 Pages: 1942-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Percutaneous coronary intervention versus coronary artery bypass grafting in patients with end-stage renal disease requiring dialysis (5-year outcomes of the CREDO-Kyoto PCI/CABG Registry Cohort-2). (2014)
  • Author(s): Marui A, Kimura T, Nishiwaki N, Mitsudo K, Komiya T, Hanyu M, Shiomi H, Tanaka S, Sakata R; CREDO-Kyoto PCI/CABG Registry Cohort-2 Investigators.
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 555-61
  • DOI: 10.1016/j.amjcard.2014.05.034.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Nakazeki F, Ide Y, Koyama S, Sowa N, Yahagi N, Shimano H, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K.
  • Journal Title: Sci Rep. Volume: 16 Pages: 5312
  • DOI: 10.1038/srep05312.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Relation of contrast-induced nephropathy to long-term mortality after percutaneous coronary intervention. (2014)
  • Author(s): Abe M, Morimoto T, Akao M, Furukawa Y, Nakagawa Y, Shizuta S, Ehara N, Taniguchi R, Doi T, Nishiyama K, Ozasa N, Saito N, Hoshino K, Mitsuoka H, Toma M, Tamura T, Haruna Y, Kita T, Kimura T.
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 362-8
  • DOI: 10.1016/j.amjcard.2014.05.009.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Anticoagulant and antiplatelet therapy in patients with atrial fibrillation undergoing percutaneous coronary intervention. (2014)
  • Author(s): Goto K, Ono K, Makiyama T, Nakagawa Y, Furukawa Y, Kadota K, Takatsu Y, Tamura T, Takizawa A, Inada T, Doi O, Nohara R, Matsuda M, Takeda T, Kato M, Shirotani M, Eizawa H, Ishii K, Lee JD, Takahashi M, Horie M, Takahashi M, Miki S, Aoyama T, Suwa S, Hamasaki S, Ogawa H, Mitsudo K, Nobuyoshi M, Kita T, Kimura T
  • Journal Title: Am J Cardiol. Volume: 114 Pages: 70-8.
  • DOI: 10.1016/j.amjcard.2014.03.060.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] AMAP1 as a negative-feedback regulator of nuclear factor-κB under inflammatory conditions. (2014)
  • Author(s): Tien DN, Kishihata M, Yoshikawa A, Hashimoto A, Sabe H, Nishi E, Kamei K, Arai H, Kita T, Kimura T, Yokode M, Ashida N.
  • Journal Title: Sci Rep. Volume: 4 Pages: 5094
  • DOI: 10.1038/srep05094.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term efficacy and safety outcomes after unrestricted use of drug-eluting stents in patients with acute coronary syndrome: mortality and major bleeding in a single-center registry. (2014)
  • Author(s): Kawaji T, Shiomi H, Morimoto T, Tamura T, Nishikawa R, Yano M, Tazaki J, Imai M, Saito N, Makiyama T, Shizuta S, Ono K, Kimura T.
  • Journal Title: Circ J. Volume: 78 Pages: 1628-35
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term clinical outcomes after everolimus- and sirolimus-eluting coronary stent implantation: final 3-year follow-up of the randomized evaluation of sirolimus-eluting versus everolimus-eluting stent trial. (2014)
  • Author(s): Shiomi H, Kozuma K, Morimoto T, Igarashi K, Kadota K, Tanabe K, Morino Y, Akasaka T, Abe M, Suwa S, Muramatsu T, Kobayashi M, Dai K, Nakao K, Uematsu M, Tarutani Y, Fujii K, Simonton CA, Kimura T; RESET Investigators.
  • Journal Title: Circ Cardiovasc Interv. Volume: 7 Pages: 343-54
  • DOI: 10.1161/CIRCINTERVENTIONS.113.001322.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two-year outcome of a randomized trial comparing second-generation drug-eluting stents using biodegradable or durable polymer. (2014)
  • Author(s): Natsuaki M, Kozuma K, Morimoto T, Shiomi H, Kimura T.
  • Journal Title: JAMA. Volume: 311 Pages: 2125-7
  • DOI: 10.1001/jama.2014.3584.
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] miR-33a/bによる細胞・臓器レベルの脂質代謝制御機構 (2015)
  • Author(s): 尾野 亘
  • Organizer: 第44回日本心脈管作動物質学会
  • Place of Presentation: 高松
  • Year and Date: 2015-02-07 – 2015-02-07
  • Invited
• [Presentation] Microrna-33, Located Within Srebf2 Intron, Regulate Fatty Acid Synthesis via Targeting SREBP-1 in vivo (2014)
  • Author(s): 1)Nishino T, Horie T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Ide Y, Nakazeki F, Koyama S, Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: American Heart Association Annual Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] MicroRNA-33b Knock-in Mice for an Intron of Sterol Regulatory Element-Binding Factor 1 (Srebf1) Exhibit Reduced HDL-C in vivo (2014)
  • Author(s): 2)Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Ide Y, Nakazeki F, Koyama S, Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: American Heart Association Annual Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Serum HDL-C decreases in microRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1). (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakano T, Nishiga M, Usami S, Izuhara M, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-33 deficiency reduces atherosclerotic plaque progression in apoE knockout mice. (2014)
  • Author(s): Baba O, Horie T, Ono K, Kuwabara Y, Sowa N, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-451 is involved in diabetic cardiomyopathy through suppression of the LKB1/AMPK pathway. (2014)
  • Author(s): Kuwabara Y, Horie T, Baba O, Nishiga M, Usami S, Izuhara M, Nakao T, Nishino T, Kita T, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-33, embedded in Srebf2 intron, regulate fatty acid synthesis through targeting SREBP-1 in vivo. (2014)
  • Author(s): Nishino T, Horie T, Baba O, Kuwabara Y, YOkode M, Kita T, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] microRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. (2014)
  • Author(s): 尾野 亘、堀江貴裕、西野共達、北 徹、木村 剛
  • Organizer: 第5回 Molecular Cardiovascular Conference Ⅱ
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] 2)飽和脂肪酸により誘導されるmicroRNA-451は、糖尿病性心筋症の増悪因子である。 (2014)
  • Author(s): 桑原康秀、堀江貴裕、馬場 理、出原正康、宇佐美俊輔、中尾哲史、西賀雅隆、西野共達、井手裕也、中関典子、小山智史、木村 剛、尾野 亘
  • Organizer: 第5回 Molecular Cardiovascular Conference Ⅱ
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] MicroRNA-33 deficiency leads to high fat diet- induced obesity and insulin resistance in vivo. (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakano T, Nishiga M, Usami S, Izuhara M, Kimura T, Ono K.
  • Organizer: European Society of Cardiology Congress 2014
  • Place of Presentation: Barcerona
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] MicroRNA-33, embedded in Srebf2 intron, regulate fatty acid synthesis through targeting SREBP-1 in vivo. (2014)
  • Author(s): Nishino T, Horie T, Baba O, Kuwabara Y, YOkode M, Kita T, Kimura T, Ono K.
  • Organizer: European Society of Cardiology Congress 2014
  • Place of Presentation: Balcerona
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] microRNA-33の動脈硬化・脂質制御における役割 (2014)
  • Author(s): 尾野 亘
  • Organizer: 第46回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-10 – 2014-07-10
  • Invited
• [Presentation] High impact of microRNA-33 on atherosclerosis and lipid metabolism (2014)
  • Author(s): 尾野 亘
  • Organizer: 第46回日本動脈硬化学会総会・学術集会 第9回五島雄一郎賞受賞講演
  • Place of Presentation: 東京
  • Year and Date: 2014-07-10 – 2014-07-10
  • Invited
• [Presentation] MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein-2 (Srebp2) regulates Srebp1 in vivo (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Hasegawa K, Kume N Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2014-04-14 – 2014-04-14
• [Presentation] MicroRNA-33 deficiency reduces atherosclerotic plaque progression in apoE knockout mice (2014)
  • Author(s): Baba O, Horie T, Kuwabara Y, Chujo Y, Watanabe S, Kinoshita M, Horiguchi M, Nakamura T, Chonabayashi K, Hishizawa M, Hasegawa K, Kume N Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2014-04-14 – 2014-04-14
• [Presentation] SREBP-2のイントロンに存在するマイクロRNA-33はSREBP-1の発現を制御する (2014)
  • Author(s): 堀江貴裕
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11 – 2014-04-11
• [Presentation] microRNA-451はLKB1-AMPK経路を抑制することで、ラット心筋細胞において細胞障害を、生体においては高脂肪食誘導性心肥大を誘導する (2014)
  • Author(s): 桑原康秀
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11 – 2014-04-11
• [Presentation] アポE欠損マウスにおいてマイクロRNA-33欠損は動脈硬化進展を抑制する (2014)
  • Author(s): 馬場 理
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11 – 2014-04-11
• [Book] Annual Review 循環器2014 (2014)
  • Author(s): 尾野 亘
  • Total Pages: 315
  • Publisher: 中外医学社
• [Remarks] 京都大学医学部付属病院循環器内科
  • URL: http://kyoto-u-cardio.jp/