Attempt to regenerate hair follicles using human iPS cell-derived epithelial and mesenchymal cells optimized for trichogenicity

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyorin University (2015); Keio University
• Principal Investigator: Ohyama Manabu 杏林大学, 医学部, 教授 (10255424)
• Co-Investigator(Kenkyū-buntansha): MASAYUKI AMAGAI 慶應義塾大学, 医学部, 教授 (90212563) ; AKIHARU KUBO 慶應義塾大学, 医学部, 講師 (70335256)
• Co-Investigator(Renkei-kenkyūsha): HIDEYUKI OKANO 慶應義塾大学, 医学部, 教授 (60160694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 毛包再生 / ヒトiPS細胞 / 分化誘導 / 上皮ー間葉系相互作用

Outline of Final Research Achievements

The aim of this study is to generate hair follicle structure using human induced pluripotent stem cell (iPSC)-derived epithelial and mesenchymal components optimized for trichogenicity. With regards to epithelial cells, a stable protocol to induce keratinocytes containing stem cell marker expressing cells was established. For trichogenic mesenchymal component preparation, an approach to generate multipotent mesenchymal stem cell-like cells was developed. The resultant cells are further programmed to acquire hair inductive property of dermal papilla cells. When co-grafted with normal human keratinocytes into immunedeficient mice, those cells gave rise to hair follicle-like structures with moderate reconstitution efficiency. However, regeneration of hair follicle structure exclusively consisted of human iPSC-derived cells is still technically very challenging, mainly because of relatively low efficiency and adjustment of the timing in the production of both iPSC-derived subsets.

Free Research Field

皮膚科学