A transgenic-cloned pig model expressing fluorescent or non-fluorescent modified Plum gene for the evaluation of islet transplantation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293279
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Meiji University
• Principal Investigator: Masaki Nagaya 明治大学, 公私立大学の部局等, 教授 (90329300)
• Co-Investigator(Kenkyū-buntansha): NAGASHIMA HIROSHI 明治大学, 農学部, 教授 (50318664) ; UMEYAMA KAZUHIRO 明治大学, 研究・知財戦略機構, 特任准教授 (70342699) ; WATANABE MASATO 明治大学, 研究・知財戦略機構, 特任講師 (00321688) ; MATSUNARI HITOMI 明治大学, 研究・知財戦略機構, 特任講師 (70639517) ; ARAI YOSHIKAZU 明治大学, 研究・知財戦略機構, 研究推進員 (90614769)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膵ランゲルハンス島 / トランスジェニックブタ / 膵島移植 / 拒絶

Outline of Final Research Achievements

A problem with pancreatic islet transplantation for type-1 diabetes patients is the low engraftment rate of islets in the recipient. About 90% of islet transplantations are carried out by intrahepatic transplantation through the portal vein, but a variety of problems occur with this method. Frequent transplants necessary for insulin therapy withdrawal because overcoming the serious problem of transplanted islets will be impossible until new, appropriate transplant method is discovered. If two individual pigs- one that expresses fluorescent proteins, and one that expresses mutated non-fluorescent forms of these proteins- are generated using somatic cell cloning technology, the recipient pig will become immunotolerant to the donor pancreatic islet. This research was undertaken to generate such pigs and to determine appropriate sites for transplantation of islet transplantation.

Free Research Field

一般外科学