molecular mechanisms under adhesional formation after abdomonal surgery

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293280
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: Jiro Fujimoto 兵庫医科大学, 医学部, 教授 (90199373)
• Co-Investigator(Kenkyū-buntansha): KOSAKA Hisashi 兵庫医科大学, 医学部, 助教 (00532251) ; IIMURO Yuji 山梨大学, 総合研究部, 医学研究員 (30252018) ; OHASHI Koichiro 兵庫医科大学, 医学部, 病院助手 (50573987) ; NAKANISHI Kenji 兵庫医科大学, 医学部, 学長 (60172350) ; YOSHIMOTO Tomohiro 兵庫医科大学, 医学部, 教授 (60241171)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 手術後癒着 / PAI-1 / IFN-g / t-PA / NKT cell / HGF / mesothelial cell / macrophage

Outline of Final Research Achievements

We investigated and obtained results of post-opertaive adhesion formation as follows. (1)We established a mouse model of adhesion formation after hepatectomy and found overexpression of IFN-g and PAI-1, downregulation of t-PA. Moreover the same mechanisms were noted in human hepatectomy. (2)We investigated fibrogenesis relating gene expression using cultured human mesothelial cells (Met-5A and MES-F). Overexpression of a-SMA, PDGF-R, and TGF-b-R were found and the mesothelial cells itself transformed into collagen producing cells. (3)We investigated which cells accumulated into the adhesion area after mouse hepatectomy. By immune-histopathology revealed that macrophage, T-cells, and neutrophils were identified in the tissue. Thus, mesothelial cells seems to play a pivotal role in fibrogenesis in adhesion formation after surgery.

Free Research Field

外科学