Drug discovery for the treatment of severe heart failure

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293297
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Kyoto University
• Principal Investigator: Tsuneyosi Hiroshi 京都大学, 医学(系)研究科(研究院), その他 (40645412)
• Co-Investigator(Kenkyū-buntansha): SAKATA RYUZO 京都大学, 大学院医学研究科, 名誉教授 (20325781)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA JUN 京都大学, ips細胞研究所, 教授 (50335288)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 心筋疾患外科学 / iPS細胞 / 創薬スクリーニング / 心筋症

Outline of Final Research Achievements

With regards to the establishment of cardiomyopathy patient’s iPS cells, we planned to collect the myocardial tissue intraoperatively in order to recognize the characteristics of pathology. And we carried out sampling in one case. With regards to iPS cell-derived cardiac tissue sheet, we differentiated cardiomyocytes and mural cells individually and made the sheets by mixing them in strict ratio. With regards to method of drug screening, we measured extracellular electrical potentials on the multi-electrode device. We focused on the assessment of cardio toxicity and used the E4031 that caused drug-induced QT prolongation. We reproduced QT (FPD: field potential duration) prolongation and characteristic re-entry type arrhythmia visually.

Free Research Field

心臓血管外科学