Gene therapy and application of iPS cells to major organ regeneration in septic multiple organ failure

2016 Fiscal Year Final Research Report

• Project/Area Number: 25293365
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Nagoya University
• Principal Investigator: Matsuda Naoyuki 名古屋大学, 医学(系)研究科(研究院), 教授 (50332466)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 敗血症 / 多臓器不全 / 線維芽細胞 / 転写因子

Outline of Final Research Achievements

Sepsis causes fibroblast proliferation in various organs following systemic inflammation due to infection. For this process, we studied growth control of fibroblasts in mouse sepsis model and human fibroblast cell culture system. Male BALB-C mice of 8 to 12 weeks as a sepsis animal model were used to evaluate pathology by cecal ligation and puncture (CLP). Analysis of the distribution of fibroblasts using S100 calcium-binding protein, etc. showed an increase in fibroblasts in the lungs and the atrial fascia interface in the time course after CLP. In the study of cell culture of human fibroblast IMR-90, it was possible to evaluate that the β receptor activation by catecholamine and thrombin activity in systemic inflammation are strongly involved in fibroblast proliferation. It was revealed that fibroblast proliferation and organization was observed in association with inflammation of sepsis in the lung and heart.

Free Research Field

救急医学