Subpopulation of dental pulp stem/progenitor cells: Their implication in the differentiation capacity, origin, and microenvironment

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293371
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824)
• Co-Investigator(Kenkyū-buntansha): TSUJIGIWA Hidetsugu 岡山理科大学, 理学部, 教授 (70335628) ; HONDA Masaki 愛知学院大学, 歯学部, 教授 (70361623)
• Co-Investigator(Renkei-kenkyūsha): IDA Hiroko (YONEMOCHI Hiroko) 新潟大学, 医歯学系, 准教授 (60293213) ; NAKATOMI Mitsushiro 九州歯科大学, 歯学部, 講師 (10571771)
• Research Collaborator: SAITO Kotaro 新潟大学, 医歯学系, 助教 (10733719)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯髄 / 静的幹細胞 / 前駆細胞 / 再植・移植 / 幹細胞ニッチ / 長期ラベル細胞 / 象牙芽細胞様細胞 / TetOP-H2B-GFPマウス

Outline of Final Research Achievements

Long term label-retaining cells (LRCs) were localized in the subodontoblastic layer as well as the perivascular niche in the center of dental pulp, demonstrated by TetOP-H2B-GFP mutant mice that allow doxycycline-inducible, green fluorescent labeling of cells. The disappearance of LRCs was attributed to the extensive apoptosis occurring significantly in LRCs except for the newly-differentiated odontoblast-like cells even in cases without immunological rejection in the process of pulpal healing following allogenic tooth transplantation. Microarray and immunohistochemical analyses demonstrated that insulin-like growth factor binding protein (IGFBP) 5 is supposed to pay an important role in the maintenance of quiescent dental pulp stem/progenitor cells. Furthermore, the co-localization of transcriptional factor Gli1 and receptor Patched1 in the quiescent stem cells suggests that these cells are regulated by sonic hedgehog signaling.

Free Research Field

口腔解剖学