Comparative genome analysis useful for protecting and utilizing NPVs infecting wild silkworms from East to South Asian countries

2016 Fiscal Year Final Research Report

• Project/Area Number: 25304047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Insect science
• Research Institution: Yamaguchi University
• Principal Investigator: Kobayashi Jun 山口大学, 創成科学研究科, 教授 (70242930)
• Co-Investigator(Kenkyū-buntansha): 門野 敬子 国立研究開発法人農業生物資源研究所, 昆虫科学研究領域, ユニット長 (40355722)
• Research Collaborator: SHIMIZU Osamu 元群馬県蚕糸技術センター ; KAJIURA Zenta 信州大学, 繊維学部, 教授 ; WANG Xueyin 瀋陽農業大学, 教授 ; HUANG Yuanjiao 広西医科大学, 教授 ; SUSAMTO Somwiharjo ガジャマダ大学, 教授
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 核多角体病ウイルス / 野蚕 / ウイルスゲノム / 昆虫病理 / 昆虫機能利用

Outline of Final Research Achievements

Nucleopolyhedroviruses (NPVs) have been isolated from various saturniid silkmoths (wild silkworms) utilized for silk production from East to South Asian countries, and their whole genome DNA sequences have been analyzed and compared using Next-Generation sequencer. As a result, a mutiplex-PCR diagnostic protocol, which enables to detect each NPV with high sensitivity and specificificity, has been successfully developed and useful knowledge in construction of baculovirus expression vector system with advantageous properties for valuable protein production and in consideration of genetic variation and evolution of NPVs infecting wild silkworms have been obtained.

Free Research Field

分子昆虫学