Exploration of esophageal cancer etiology using comprehensive DNA adduct analysis (DNA adductome analysis)

2015 Fiscal Year Final Research Report

• Project/Area Number: 25305026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: National Cancer Center Japan
• Principal Investigator: Totsuka Yukari 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (40373401)
• Co-Investigator(Kenkyū-buntansha): Lin Yingsong 愛知医科大学, 医学部, 准教授 (50340302)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アダクトーム

Outline of Final Research Achievements

Ci Xian, one of the high-risk areas in China, demonstrates a much higher incidence when compared with other urban areas in China. To address the etiology of esophageal cancer in Ci Xian, we carried out a comprehensive DNA adduct analysis (DNA adductome) using surgical specimens collected from esophageal cancer patients living in high- and low-risk areas. The results of principal component analysis showed that THP-dG, which was derived from N-nitrosopiperidine (NPIP), emerged as a major DNA adduct. In order to confirm the relationship between NPIP and esophageal cancer development, the THP-dG levels using biological samples collected from subjects residing in both high- and low-risk areas were analyzed. Levels and detection rates of THP-dG seemed higher in the high-risk area compared to those of the low-risk area.

Free Research Field

化学発がん