2016 Fiscal Year Final Research Report
A study of the relationship between DNA methylation profile of promoter region and gene expression
| Project/Area Number |
25330347
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | がん / 遺伝子発現 / マイクロアレイ / DNAメチル化 / 転写制御 |
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| Outline of Final Research Achievements |
The object of analysis in this study is DNA methylation. DNA methylation is a phenomenon in which a methyl group is added to a CpG site. When DNA methylation occurs, binding of transcription factors is inhibited, and transcription of genes is suppressed.
A DNA methylation inhibitor was added to human pulmonary artery endothelial cells and the gene expression change was measured. DNA methylation analysis of genes with altered expression was also performed. There was almost no change in the degree of DNA methylation of the gene whose expression was greatly changed. From this result, it is considered that DNA methylation does not regulate gene expression on its own.
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| Free Research Field |
バイオインフォマティクス
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