Identification of the specific receptors for the nanomaterials

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Shinshu University
• Principal Investigator: USUI Yuki 信州大学, 医学部附属病院, 特任研究員 (00467169)
• Co-Investigator(Kenkyū-buntansha): HANIU Hisao 信州大学, 学術研究院医学系, 准教授 (30252050) ; TSUKAHARA Tamotsu 長崎大学, 医歯(薬)学総合研究科, 准教授 (00529943)
• Co-Investigator(Renkei-kenkyūsha): SAITO Naoto 信州大学, 学術研究院保健学系, 教授 (80283258) ; KOBAYASHI Shinsuke 信州大学, 医学部附属病院, 医員 (40624705) ; NOMURA Hiroki 信州大学, 医学部附属病院, 医員 (40646543)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生体物性 / ナノマテリアル / カーボンナノチューブ / エンドサイトーシス

Outline of Final Research Achievements

The culture medium composition affected the proteins that are expressed on the cytoplasmic membrane, which influence the biological response to MWCNTs, especially its cellular uptake. MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways (clathrin-mediated, caveolae-mediated, and macropinocytosis). ITGB1 and MARCO may related to receptor-mediated endocytosis of MWCNTs in the nonphagocytic cells. The MWCNTs-endocytosed cells induced LC3B expression and induced cell growth inhibition.

Free Research Field

生体材料学