The effect of iPS cell- and bFGF drug- delivery system on bioabsorbable nerve conduits in peripheral nerve regeneration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350537
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Osaka City University
• Principal Investigator: Nakamura Hiroaki 大阪市立大学, 大学院医学研究科, 教授 (60227931)
• Co-Investigator(Kenkyū-buntansha): OKADA Mitsuhiro 大阪市立大学, 大学院医学研究科, 講師 (40309571) ; UEMURA Takuya 大阪市立大学, 大学院医学研究科, 病院講師 (10597321) ; TAKAMATSU Kiyohito 大阪市立大学, 大学院医学研究科, 客員准教授 (30295688)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: iPS細胞 / 人工神経 / 末梢神経 / 再生医療 / 線維芽細胞増殖因子 / ドラッグデリバリーシステム / 成長因子

Outline of Final Research Achievements

For peripheral nerve repair, various modifications have been explored. Here, sciatic nerve gaps in mice were reconstructed in the following groups: nerve conduit alone (C group), nerve conduit coated with induced pluripotent stem cell (iPSc)-derived neurospheres (I group), nerve conduit coated with iPSc-derived neurospheres and basic fibroblast growth factor (bFGF)-incorporated gelatin microspheres (I+F group), and autograft (A group). The fastest functional recovery and the greatest axon regeneration occurred in the A group, followed in order by the I+F group, I group, and C group until 12 weeks after reconstruction. Thus, peripheral nerve regeneration using nerve conduits and functional recovery in mice were accelerated by a combination of iPSc-derived neurospheres and a bFGF drug delivery system. The combination of all three fundamental methodologies was essential and useful for peripheral nerve regenerative medicine.

Free Research Field

整形外科