Different integrins are essential to the specific responses of bone cells to variable mechanical stimuli

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350542
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kanagawa Dental College
• Principal Investigator: Mikuni-Takagaki Yuko 神奈川歯科大学, 歯学研究科(研究院), 特任教授 (60050689)
• Co-Investigator(Kenkyū-buntansha): TANAKA Takahiro 神奈川歯科大学, 歯学部, 研究員 (90550830)
• Co-Investigator(Renkei-kenkyūsha): MORISHITA Shin 横浜国立大学, 研究院環境情報研究科, 教授 (80166404)
• Research Collaborator: HARRISON Andrew
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨細胞（osteocyte） / 骨芽細胞（osteoblast） / 力学刺激 / インテグリン / 細胞分化

Outline of Final Research Achievements

Previous studies of our own and others showed that α5β1 integrin is involved in the mechanical response of osteoblasts to low-intensity pulsed ultrasound (LIPUS) and αvβ3 integrin is involved in that of osteocytes to stretching, respectively. In this study, we further show that differentiation of osteoblasts to osteocytes was accelerated by LIPUS resulting in the increased expression of αvβ3 integrin. Treating isolated osteoblasts with neutralizing antibody to α5β1 integrin prior to the LIPUS exposure inhibited both mechanical response to LIPUS and the increased expression ofαvβ3 integrin. Prior treatment with the inhibitor of Mitogen-Activated Protein Kinase ERK1/2, PD98059, however, did not. LIPUS prevents apoptosis and induce bone remodeling also through α5β1 integrin by the increased expression of BCl-2 and RANKL in the mandibular osteoblasts, in which mechanical loading is essential for homeostasis.

Free Research Field

硬組織と力学刺激