Development of genetically-encoded multi-color ATP sensors for clarification of energy metabolism.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25350970
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Waseda University
• Principal Investigator: KITAGUCHI TETSUYA 早稲田大学, 総合研究機構, 主任研究員(研究院准教授) (60432374)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: GFP

Outline of Final Research Achievements

Adenosine triphosphate (ATP) is an energy currency for living organisms. Therefore, we are able to clarify the mechanism of energy metabolism by visualizing ATP dynamics in the living cells. To achieve this objective, we developed a genetically-encoded fluorescent indicator for ATP. These indicators are single-fluorescent protein (FP) based, therefore change their intensity respond to ATP. We employed a green FP variant, Citrine as transmitter domain and a subunit of ATP-binding region on the bacterial FoF1-ATP synthase as sensing domain. Likewise, we employed blue FP and mApple to develop blue or red emitted indicators, respectively. Based on these indicators, we have monitored ATP dynamics in living cells by multi-color imaging.

Free Research Field

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