2015 Fiscal Year Final Research Report
Development of a new two-dimensional in-gel phosphatase assay and its application to protein phosphatase research
| Project/Area Number |
25350978
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUEYOSHI NORIYUKI 香川大学, 農学部, 准教授 (90346635)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 細胞内情報伝達 / タンパク質リン酸化 / プロテインホスファターゼ / カルモジュリンキナーゼ / 翻訳後修飾 / プロテインキナーゼカスケード |
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| Outline of Final Research Achievements |
Two major objectives of this research project are as follows. (1) Development of a new two-dimensional in-gel phosphatase assay. (2) Elucidation of the regulatory mechanisms of PPM family phosphatases including CaM-kinase phosphatase (CaMKP) and nuclear CaMKP (CaMKP-N). In this project, we have successfully developed a unique two-dimensional in-gel phosphatase assay using fluorogenic substrate, 4-methylumbelliferyl phosphate (MUP). Futhermore, we clarified the regulatory mechanisms of cellular localization of CaMKP-N through proteolytic processing and found a new anchoring protein, protocadherin gamma subfamily C5, that regulate CaMKP activity in vivo.
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| Free Research Field |
生物化学
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