Development of a hybrid microscope to observe inner structures and their variation of living biological cells in situ.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25390134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: KADO Masataka 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究主幹 (30360431)
• Co-Investigator(Renkei-kenkyūsha): KISHIMOTO Maki 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究主幹 (40360432)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 軟X線顕微鏡 / 相関顕微法 / 細胞内小器官 / アポトーシス / 生きている細胞

Outline of Final Research Achievements

I have developed a hybrid microscopy which is a combination of a fluorescent microscope and a soft X-ray microscope and this microscopy enabled to observe live biological cells with both microscopes at the same time. Observing Leydig cells of mouse testis with the hybrid microscopy, I have succeeded to obtain a soft X-ray image of a single mitochondrion in a live biological cell. The hybrid microscopy also was applied to observe apoptotic cells and details of structural deformation due to the apoptosis have been cleared.

Free Research Field

X線光学