2015 Fiscal Year Final Research Report
Regulation of photochemical reaction by grapheme oxide and its application to gene analysis
| Project/Area Number |
25410146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | DNA / グラフェン / バイオセンサー / 遺伝子解析 |
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| Outline of Final Research Achievements |
Partially oxidized graphene (graphene oxide, GO) is an atomically thin, two-dimensional sheet that contains nanometer-scale graphene-like sp2 domains in an amorphous sp3 carbon matrix. It can strongly adsorb single-stranded DNA (ssDNA) via non-covalent hydrophobic and π-stacking interactions with the exposed nucleobases. Meanwhile, GO affinity for double-stranded DNA is much lower because the nucleobases are shielded inside the hydrophilic double helix backbone. GO is also a very effective fluorescence quencher owing to its efficient, long-range energy transfer properties.
In this research, fluorescent-dye-labeled probe DNA was immobilized on fluorescence-quenching graphene oxide (GO) through a capture DNA. When targets were added, the probes were released from the GO through toehold-mediated strand exchange. Higher emission recovery and more signal contrast were achieved relative to conventional methods that are based on direct adsorption of probes.
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| Free Research Field |
分析化学
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