Development of the production system of functional carotenoids rare in nature using chloroplast-genome modified lettuce

2016 Fiscal Year Final Research Report

• Project/Area Number: 25410177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: MISAWA Norihiko 石川県立大学, 生物資源環境学部, 教授 (30393466)
• Co-Investigator(Renkei-kenkyūsha): MAOKA Takashi 一般財団法人生産開発科学研究所, 食物機能研究室, 室長 (10157159)
• Research Collaborator: TAKEMURA Miho
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: カロテノイド / レタス / 葉緑体形質転換 / カプサンチン / アスタキサンチン / フリチエラキサンチン / ノストキサンチン / イドキサンチン

Outline of Final Research Achievements

Carotenoids are one of the representative natural pigments, and prevent human body from oxidative stress. As promising functional carotenoids rare in nature, we noted 4-Ketocapsanthin, 2,2’-Dihydroxyastaxanthin, and Nostoxanthin. Plasmids for the biosynthesis of these pigments in the chloroplasts were constructed, and used to obtain respective chloroplast-genome modified (CGM) lettuce plants. Pigment analysis was carried out on these CGM lettuce plants. CGM lettuce that synthesized ketocarotenoids such as Astaxanthin and Fritschiellxanthin (4-Ketolutein; promising functional carotenoids rare in nature), which had already constructed in the beginning of the project, were cultivated with large scale, and supplied for experiments on plant physiology and confirmation study as food safety.

Free Research Field

合成生物学