2015 Fiscal Year Final Research Report
G-quadruplex formation controlled by irradiation of triphenylmethane derivatives
| Project/Area Number |
25410185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Nara National College of Technology |
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Principal Investigator |
Uda Ryoko 奈良工業高等専門学校, その他部局等, 准教授 (90321463)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 光応答 / グアニン四重鎖 / トリフェニルメタン |
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| Outline of Final Research Achievements |
Photoresponsive malachite green copolymer has been designed to induce the formation of G-quadruplex by light. The interaction of the copolymer with d[(TTAGGG)4] oligonucleotide has been investigated by fluorescence analysis. Circular dichroism spectroscopies revealed that irradiation of the mixture of the copolymer and d[(TTAGGG)4] leads to the formation of antiparallel G-quadruplex. The results of competition dialysis assay indicated that the binding of malachite green to G-quadruplex of d[(TTAGGG)4] was selective. The G-quadruplex structure was found to be stabilized by the binding of malachite green.
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| Free Research Field |
有機化学
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