Study on Improvement of Gene Transfer Efficiency with Pulsed Power Technology

2015 Fiscal Year Final Research Report

• Project/Area Number: 25420276
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Power engineering/Power conversion/Electric machinery
• Research Institution: Ariake National College of Technology
• Principal Investigator: Kono Susumu 有明工業高等専門学校, その他部局等, 准教授 (30270375)
• Research Collaborator: TOMINAGA NOBUAKI ; YAMAGUCHI AKEMI ; Takehito Kaneko
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: パルスパワー / エレクトロポレーション / バーストパルス / 遺伝子 / ＧＦＰ / 受精卵 / メダカ / 物質導入

Outline of Final Research Achievements

We have assembled the asymmetric burst pulse system that can generate a single rectangular high voltage pulse in nanosecond and some low voltage burst pulses with both polarities from microsecond to millisecond. It was able to introduce some chemical substances into fertilized medaka eggs easily and efficiently with this pulse system. As a next challenge, a preparation of a transgenic organism with the pulse system was studied. Green Fluorescent Protein (GFP) plasmid vector was tried to introduce into fertilized medaka eggs by this system. GFP expression was observed in embryo at some pulse parameters, indicating that plasmid vector was transferred to medaka embryo cell and expressed coding GFP successfully. It became clear that the pulse shapes of both the pre- and the post-burst pulses and the length of H.V. short pulse were important parameters to induce the gene transfer. It is thought that this asymmetric burst pulse system would become a simple and easy incorporation system.

Free Research Field

パルスパワー