2015 Fiscal Year Final Research Report
Development of microbial catalyst for biomass refinery based on syngas platform
| Project/Area Number |
25420832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 水素 / 二酸化炭素 / 合成ガス / エタノール / 発酵 / ホモ酢酸菌 / 代謝工学 |
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| Outline of Final Research Achievements |
In this study, we aimed to evaluate the availability of useful metabolite production by means of synthetic biotechnology from syngas by Moorella thermoacetica as a host microorganism. After developing novel transformation system of Moorella with thermotolerant kanamycin resistant marker gene, we successfully constructed a mutant strain that mainly produced ethanol from fructose by the disruption of acetate synthetic pathway and introduction of alcohol synthetic pathway. Although the mutant lost fermentability of H2-CO2, it was recovered by the addition of sugar, suggesting that the mutant is a promising candidate for ethanol production by co-fermentation of carbohydrate and syngas.
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| Free Research Field |
生物化学工学
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