Functional roles of glial ATP receptors in neuron-glia interaction

2016 Fiscal Year Final Research Report

• Project/Area Number: 25430003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Gunma University
• Principal Investigator: HOSOI Nobutake 群馬大学, 大学院医学系研究科, 講師 (90543570)
• Co-Investigator(Renkei-kenkyūsha): KONNO Ayumu 群馬大学, 大学院医学系研究科, 講師 (40509048) ; HIRAI Hirokazu 群馬大学, 大学院医学系研究科, 教授 (70291086)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: AAV / 小脳 / グリア / 二重感染

Outline of Final Research Achievements

Similarly to neurons, glial cells have ATP receptors and may play an active role in neuronal information processing. Originally, in order to examine functional roles of cerebellar glial ATP receptors, I tried to knockdown glial ATP receptors using microRNA-mediated gene silencing technique and it did not work. However, while I tried to express transgenes selectively in cerebellar glial cells with adeno-associated virus (AAV) vectors, I managed to develop a method in which larger transgenes can be expressed in glial cells, compared to the usual usage of AAV vectors. The pros and cons of this method were examined experimentally, and I am preparing for publishing the results.

Free Research Field

神経科学