2015 Fiscal Year Final Research Report
Molecular and physiological mechanisms of the cerebellar compartmentalization
| Project/Area Number |
25430008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Fukushima Medical University (2015)
Nagoya University (2013-2014) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 小脳 / アデノウイルスベクター / 神経細胞の誕生日 / 22q13欠失症候群 / Wnt7b |
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| Outline of Final Research Achievements |
1. We newly generated an adenoviral vector expressing antisense RNA of Wnt7b. The adenoviral vector suppressed successfully Wnt7b-expression in vitro and in vivo. Using the adenoviral vector, we suppressed Wnt7b-expression in neurons born on embryonic day (E)11.5, selectively. Wnt7b-suppression caused underdevelopment, neocortex hypoplasia, cerebellar vermis hypoplasia, autism-like abnormal behavior, and abnormal EEG.
2. We newly made two kinds of optical-stimulators that were small enough to mount the device on a mouse head.One was controlled by infrared wireless communication and another one was controlled bywireless communication of Bluetooth 4.0 (Bluetooth Low Energy) . The Bluetooth-device could be modulated by an iPad or iPhone application.
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| Free Research Field |
神経科学
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