Functional analysis of CRMP4 in the developing olfactory bulb and hypothalamus

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Toyo University
• Principal Investigator: Ohtani-Kaneko Ritsuko (大谷律子) 東洋大学, 生命科学部, 教授 (00161183)
• Co-Investigator(Kenkyū-buntansha): Goshima Yoshio 横浜市立大学, 医学研究科, 教授 (00153750) ; Nishihara Masugi 東京大学, 農学生命科学研究科(農学部), 教授 (90145673)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: CRMP4 / ノックアウトマウス / 神経回路 / 嗅球

Outline of Final Research Achievements

Our previous study revealed that CRMP4 is one of candidate proteins involving in the sexual differentiation of sexually dimorphic nucleus (AVPV) (Iwakura et al., 2013). In this study, morphological analysis of the olfactory bub (OB) in Crmp4-KO mice showed a new role for CRMP4 in the regulation of dendritic elongation and the determination of certain layer thickness. Furthermore, focusing on the olfaction process, we found impaired olfactory ability in Crmp4-KO pups. In addition, the study on c-Fos expression demonstrated hyperactivity in the OB of Crmp4-KO pups compared with WTs when both pups were exposured to one odorant. Since the mRNA and protein expression levels of GluR1 and GluR2 were exaggerated in Crmp4-KO pups, it is implied that enhanced expression of these excitatory receptors contributes to the hyperactivity of neurons in the OB and the impaired olfactory ability. The results of this study provide much suggestive information on the function of CRMP4.

Free Research Field

神経発生学