Phosphorylated recombinant HSP27 as a new neuroprotective agent for acute ischemic stroke

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Juntendo University
• Principal Investigator: TANAKA RYOTA 順天堂大学, 医学部, 准教授 (40407284)
• Co-Investigator(Kenkyū-buntansha): SHIMURA Hideki 順天堂大学, 医学部, 准教授 (50286746)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Nobutaka 順天堂大学, 医学部, 教授 (80218510)
• Research Collaborator: SHIMADA Yoshiaki 順天堂大学, 医学部, 助教 (10761455)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: HSP27 / リン酸化 / 脳梗塞急性期 / 脳保護療法 / tPA / 血管内皮保護

Outline of Final Research Achievements

We studied the effect of activated (phosphorylated) recombinant HSP27 (prHSP27) as a new neuroprotective agent for acute ischemic stroke. Intravenous administration of prHSP27 resulted in the significant reduction of infarct volume and the improvement of neurological deficit than control in mice 24 hours after transient focal ischemia. When the thrombolytic agent tissue plasminogen activator (tPA) was co-administered, prHSP27 also attenuated the blood-brain barrier disruption significantly and the hemorrhagic transformation than control. Intravenous administration of prHSP27 could reduce the excessive oxidative stress, inflammation, apoptosis and neurovascular injury after focal cerebral ischemia. Activated recombinant HSP27 is useful as a new neuroprotective agent for acute stage of ischemic stroke.

Free Research Field

神経内科学