2015 Fiscal Year Final Research Report
Development of novel humanized mouse model recapiturated human allergic responses.
| Project/Area Number |
25430099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
Ito Ryoji 公益財団法人実験動物中央研究所, その他部局等, 研究員 (60425436)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ヒト化マウス / アレルギー |
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| Outline of Final Research Achievements |
A purpose of this study is to develop human allergy model using humanized mice. We evaluated human allergic responses by PCA reaction usinghumanized hIL-3/GM-CSF Tg mice, which can be fully differentiated human mast cells and basophils, after administration of the serum from pollinosis patients and pollen extract.Human mast cell-mediated allergic reaction efficientry developed in the skin of Tg mice but not of nonTg mice. Furthermore, we established novel humanized mice introduced hIL-5 gene into IL-3/GM Tg mice. In lungs of these triple-Tg mice, human eosinophils has infiltrated after intratracheal administration of HDM. Therefore, these humanized mice may contribute to develop the new anti-allergic drugs.
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| Free Research Field |
免疫学
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