Precancerous signatures explored through micro-genomics and micro-proteomics

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Nihon University
• Principal Investigator: ESUMI Mariko 日本大学, 医学部, 准教授 (10147019)
• Co-Investigator(Kenkyū-buntansha): SUGITANI Masahiko 日本大学, 医学部, 教授 (40187654)
• Co-Investigator(Renkei-kenkyūsha): TAKAYAMA Tadatoshi 日本大学, 医学部, 教授 (30280994) ; KURODA Kazumichi 日本大学, 医学部, 准教授 (50215109) ; YAMAGUCHI Hiromi 日本大学, 医学部, 研究員 (90547118)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: レーザーマイクロダイセクション / プロテオミクス / 微小環境 / 再発肝癌 / ゲノミクス / 次世代シーケンサー / ホルマリン固定パラフィン包埋切片

Outline of Final Research Achievements

We investigated comprehensive profiles of genome and proteome in precancerous, cancerous lesions and stroma cells from early and late recurrence groups to identify the molecular difference among them, and finally to elucidate the molecular genesis and progression of cancer. In conclusion, (1) microenvironment of parenchyma such as fibrosis and inflammation was related to hepatocarcinogenesis and intra-hepatic metastasis. (2) Abnormal lesions but not cancer (dysplasia) shared mutation with cancerous lesion, indicating that dysplasia is molecularly precancerous. Multi-centric carcinogenesis occurred from a single clonal dysplasia by different gene mutations. (3) Early cancer less than 2 cm in diameter contained different cancer clones originated from a single clone, indicating that branched evolution of cancer begins from the early stage of cancer. (4) Relatively small number of driver gene mutation was involved in hepatocarcinogenesis of rat.

Free Research Field

実験病理学 病態医化学 ウイルス学 腫瘍生物学 ゲノム医科学 腫瘍診断学