Contribution of tRNA-splicing machinery in development of permuted and intronic tRNA genes.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Chiba University
• Principal Investigator: Akiko Soma 千葉大学, 園芸学研究科, 助教 (70350329)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: RNAプロセシング / 翻訳

Outline of Final Research Achievements

C. merolae nuclear genome contains number of intronic tRNA genes and circularly permuted tRNA genes. Precursors derived from both tRNA genes contain the BHB motif, which is the primal recognition site for the tRNA-splicing enzyme. Terefore, both tRNAs are considered to be processed by tRNA-splicing machinery and be evlutionaly correlated each other. This study showed that the permuted tRNA can be developed from tandemly repeated intronic tRNA genes in C. merolae cells. We also found that variously disrupted tRNA genes can be expressed in C. merolae cells probably because of the noncanonical tRNA maturation pathway.

Free Research Field

分子生物学