Structural characterization of Hfq-binding small regulatory RNA

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: MORITA Teppei 鈴鹿医療科学大学, 薬学部, 助手 (10444366)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 小分子RNA / 転写後制御 / Hfq / 大腸菌

Outline of Final Research Achievements

This study is aimed to clarify the structural feature of bacterial small regulatory RNA (sRNA). The sRNAs repress target mRNAs by forming duplex in Escherichia coli as in many other species. The duplex formation between sRNA and target mRNA is accelerated by RNA chaperon protein, Hfq. The major outcome of this study is that the functional Hfq-binding module must be located at the 3’ end of sRNA. It is also shown that transcription termination is enhanced under stress conditions in which transcription of sRNAs are induced. These findings are useful for understanding of how the functional sRNAs are generated in bacterial cells.

Free Research Field

生物学