The molecular mechanism for fundamental properties of circadian clock, input, oscillation, and output

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: YOSHITANE Hikari 東京大学, 理学(系)研究科(研究院), 助教 (70569920)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: サーカディアンリズム / 体内時計 / ChIP-Seq解析 / RNA-Seq解析 / インタラクトーム解析

Outline of Final Research Achievements

We previously identified CLOCK-binding sites in mouse liver by ChIP-Seq analysis, and in this study a newly developed bioinformatics method, motif centrality analysis of ChIP-Seq (MOCCS), revealed CACGNG, CACGTT, and CATG[T/C]G as functional E-box sequences targeted by CLOCK. We reported Nrf2 and Nfatc2 genes as rhythmic output genes from the E-boxes. Functional D-box sequences targeted by DBP protein were determined by ChIP-Seq analysis. We also identified CaMKII, UBE3A, and USP7 as a protein kinase for CLOCK phosphorylation, an E3 ligase for BMAL1 ubiquitination, and a de-ubiquitination enzyme for CRY ubiquitination, respectively.

Free Research Field

分子生物学／生化学／時間生物学