Research of phagocytosis-mediated regulation of growth rate in Drosophila

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University (2014-2015); Kanazawa University (2013)
• Principal Investigator: NAGAOSA Kaz 弘前大学, 食料科学研究所, 准教授 (70401891)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 貪食 / 成長調節 / ショウジョウバエ / 転写 / アポトーシス / 自然免疫

Outline of Final Research Achievements

Unwanted cells are induced cell death, then phagocytes recognize them using phagocytosis receptor(s) and engulfed. Our previous experiments showed that a Drosophila lacking phagocytosis receptors, Draper and integrin beta-nu, took longer time to develop into adults. This results hypothesized that phagocytosis receptor-mediated phagocytosis controlled ontogenetic growth. To be clear that, phagocytosing phagocyte was subjected to DNA microarray analysis and gel-shift assay. I found; 1) expression level of growth-related gene B was up-regulated, 2) amount of a repressor C which suppresses transcription of the gene B was decreased, and 3) another repressor A which suppresses transcription of the gene C was activated. These findings support the hypothesis.

Free Research Field

分子生物学、生化学、細胞生物学、発生生物学、免疫学