Atomic structure of cytochrome bd-type terminal oxidase in the respiratory chain and response to environment.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Sakamoto Junshi 九州工業大学, 大学院情報工学研究院, 教授 (80175364)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: シトクロム / 呼吸鎖 / 好熱性細菌 / グラム陽性菌 / 生体ネエルギー変換 / 電子伝達系 / 結晶構造 / 抗菌薬

Outline of Final Research Achievements

With improving the conditions of gene expression, host cell culture, and enzyme purification, we have succeeded in getting a highly resolved stereostructure of cytochrome bd-type menaquinol oxidase in respiratory chain of Gram-positive thermoplilic bacterium Geobacillus thermodenitirificans K1041 strain, based on a collaboration with X-ray crystallography technique by foreign researchers. This is the first atomic structure of cytochrome bd-type oxidase and does not resemble to the heme-copper oxidases or any other known proteins at all.

Free Research Field

生体エネルギー学