Folding mechanism of non-two-state proteins based on correlation analyses of kinetic folding data

2016 Fiscal Year Final Research Report

• Project/Area Number: 25440075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo (2015-2016); The Graduate University for Advanced Studies (2013-2014)
• Principal Investigator: Kuwajima Kunihiro 東京大学, 大学院理学系研究科(理学部), 名誉教授 (70091444)
• Research Collaborator: LEE Jooyoung 韓国高等科学院, 教授 ; LEE Jooyoung Korea Institute for Advanced Study, Professor ; MANAVALAN Balachandran 韓国高等科学院, 研究員 ; MANAVALAN Balachandran Korea Institute for Advanced Study, Research Fellow
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: フォールディング / 速度論 / データベース

Outline of Final Research Achievements

We carried out literature searching on the folding kinetics of globular proteins, and constructed a protein-folding database. The total entry number of the database is 141, consisting of 59 non-two-state proteins and 82 two-state proteins. We took the non-two-state proteins and the two-state proteins separately from the database, and investigated correlations between the logarithmic rate constant (ln kf) of folding and the structural properties of proteins. We show that using the logarithmic number of nonlocal contact clusters, a structural property proposed by us, we can clearly describe differences in the molecular mechanisms of folding between the non-two-state and the two-state proteins.

Free Research Field

生物物理学