Mechanism of assembly and disassembly of RNA granules responsible for mRNA transport and translational regulation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: SHIINA Nobuyuki 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 准教授 (30332175)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Kei 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (40553590)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: RNA granule / 翻訳制御 / リン酸化 / メチル化 / RNG105 / NF45 / NFAR2 / PRMT1

Outline of Final Research Achievements

The regulation of RNA granule assembly and disassembly is a structural basis of translational control. Here, we analyzed the mechanism of RNA granule assembly and disassembly regulated by NFAR2-NF45 complex and RNA granule disassembly regulated by PRMT1. NFAR2 enhanced the assembly of RNA granules, whereas NF45 promoted disassembly of RNA granules by binding to NFAR2 and inhibiting the ability of NFAR2 to assemble RNA granules. PRMT1 methylated RNG105, which reduced the ability of RNG105 to promote RNA granule assembly and resulted in the disassembly of RNA granules. These results provided novel mechanisms of assembly and disassembly of RNA granules.

Free Research Field

細胞生物学