Analysis of calcium signaling network in plants and alteration of the substrate specificity of protein kinase using plant CDPK

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Ishida Sarahmi 東京大学, 理学(系)研究科(研究院), 助教 (20282725)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 情報伝達系 / カルシウム / タンパク質キナーゼ / 基質特異性 / CDPK

Outline of Final Research Achievements

We have demonstrated that Calcium-dependent protein kinases (CDPK) in plants could be divided to two domains, that is, substrate recognition domain and catalytic domain and estimated CDPKs were suitable as the model system in analysis for altering the substrate specificity of protein kinases. The success in altering substrate specificity of protein kinases is expected to promote a novel signal transduction pathway constructed theoretically.
In this research, the mechanism for specific substrate recognition process in plant CDPK was analysed in detail aiming establishment of an examination system for altering the substrate specificity of protein kinases in signal transduction in cells.

Free Research Field

分子生物学