Linkage between DNA replication and chromosome assembly mediated by condensin II: focusing on chromosome fragile sites

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ono Takao 国立研究開発法人理化学研究所, 平野染色体ダイナミクス研究室, 専任研究員 (20291172)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: コンデンシンII / 染色体脆弱部位 / 複製ストレス / 染色体凝縮 / セントロメア / 分岐型複製中間体 / 相同組換え / 間期核ブリッジ

Outline of Final Research Achievements

Condensin II plays a central role in the process of mitotic chromosome condensation, and initiates resolution of replicated regions of chromosome s (sister chromatids) during S phase. This indicates that Condensin II is a critical factor that links chromosome duplication to condensation. To clarify the molecular mechanisms of this linkage mediate by condensin II, the role of condensin II in chromosome fragile sites (CFS), where chromatid breaks/gaps are continually observed under the presence of replicative stress, was examined in the present study. Cytological and cytogenetic assays, however, revealed that condensin II does not suppress the incidence of chromatid breaks/gaps. Alternatively, it is found that condensin II contributes to the appropriate localization of nucleases required for the process of removing a branch type replicative intermediates. This feature was evident in the process of separation in the centromeric regions under the absence of condensin II.

Free Research Field

細胞遺伝学、細胞生物学