2016 Fiscal Year Final Research Report
Genomic stability by tetraploidization in orchids and elucidation of its molecular cytogenetic mechanism
| Project/Area Number |
25450006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Osaka Kyoiku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | ラン科植物 / 四倍体 / GISH法 / 動原体 / CENH3 / メチル化 / ヒストンリン酸化抗体 / BACライブラリー |
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| Outline of Final Research Achievements |
We have elucidated the mechanism of genetic stabilization of tetraploids and amphidiploids from inter- and intra-generic hybrids in the subtribe Aeridinae of tribe Vandae which is monophyletic, mostly epiphytic and comprises horticultural valued genera including Vanda, Neofinetia, Ascocentrum, Rhynchostylis. Epigenetic chromatin dynamics by DNA methylation and histone modification were visualized at the chromosome and DNA level using the GISH and immunological staining method. As the molecular mechanism involved in the stabilization of tetraploid seems to be related to the structure of kinetochores, and as clue to unvovering it has been found by conducting the contruction of BAC library such as Neofinetia falcata and the identification of CENH3.
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| Free Research Field |
分子細胞遺伝学
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