2016 Fiscal Year Final Research Report
Cell surface expression of proteins using Pichia yeast: Development and application of high-throuput construction of expression system
| Project/Area Number |
25450126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | タンパク質工学 / Pichia pastoris / 発現系構築 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
Using High-throuput method constructing Pichia yeast protein expression system, cell surface expression of green fluorescence protein and the target protein using Pichia yeast has been achieved. Pichia yeast producing alpha-amylase on the cell surface had starch diesting activity.
Using Pichia yeast, cellulose digesting enzymes of molluscs were produced. For the enzyme of a snail, molecular structure and catalytic mechanism were analyzed.
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| Free Research Field |
農芸化学
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