Approaches to enhancement of mucosal immunity based on control of gut-type dendritic cells with retinoic acid-producing capacity

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Tokushima Bunri University
• Principal Investigator: NAKATSUMA Aya 徳島文理大学, 薬学部, 助教 (30446075)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ビタミンA / レチノイン酸 / 粘膜免疫 / 樹状細胞 / T細胞 / 免疫寛容 / アレルギー / 炎症

Outline of Final Research Achievements

GM-CSF induced retinal dehydrogenase 2 (RALDH2) and CD103 expression in bone marrow-derived dendritic cells (DCs) in the presence of retinoic acid, an active vitamin A metabolite. Subsequent activation of integrin β1 signaling enhanced DC maturation and RALDH2 expression without inducing inflammatory cytokines. The RALDH2+CD103+ DCs efficiently induced gut-tropic Foxp3+ inducible regulatory T cells and alleviated dextran sulfate sodium-induced murine colitis. Vitamin A deficiency directed intestinal epithelial cells to produce TNF-α that enhanced an ability of DCs to induce inflammatory T cells. These results suggest that retinoic acid inhibits allergic responses to oral antigens by preventing intestinal DCs from inducing inflammatory T cells.

Free Research Field

免疫学