Reproductive success of wild and hatchery-reared white-spotted charr (Salvelinus leucomaenis) based on long-term monitoring

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450290
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Japan Fisheries Research and Education Agency
• Principal Investigator: Yamamoto Shoichiro 国立研究開発法人水産総合研究センター, 増養殖研究所, 主任研究員 (20392897)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: イワナ / マイクロサテライトDNA / 繁殖成功度 / 親子判別

Outline of Final Research Achievements

Tri-and tetra-motif repeat microsatellite marker loci were developed for the white-spotted charr (Salvelinus leucomaenis). The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite-primer sets, available for the estimation of polymorphisms, were identified. To evaluate individual reproductive success of white-spotted charr, I collected nine consecutive years DNA samples of white-spotted charr in a wild population. Both males and females exhibited greater variances in reproductive success among individuals, 0-29% (min.-max. during 2005-2013) of the mature males were assigned fatherhood of the offspring, and 9-100% of the mature females were assigned motherhood of the offspring (full-pedigree likelihood method). I also examined reproductive success of hatchery-reared white-spotted charr in a semi-natural artificial stream. Hatchery-reared fish also exhibited a large individual variation in reproductive success in both males and females.

Free Research Field

魚類生態遺伝学